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Biosset Ltd asm-102u dna synthesizer
Asm 102u Dna Synthesizer, supplied by Biosset Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/asm-102u+dna+synthesizer/asm+800+dna+rna+synthesizer/pmc03956407-70-6-8
Average 90 stars, based on 1 article reviews
asm-102u dna synthesizer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Synthesized:

Article Title: A genome-wide sequence-independent comparative analysis of insertion-deletion polymorphisms in multiple Mycobacterium tuberculosis strains.
Article Snippet: .. Oligonucleotides were synthesized using an ASM-102U DNA synthesizer (Biosset Ltd., Russia). .. Purified genomic DNA samples of the two comparable strains (0.5 μg each) were digested with AluI (MBI Fermentas, Lithuania).

Article Title: Recognition of base pair inversions in duplex by chimeric (alpha,beta) triplex-forming oligonucleotides.
Article Snippet: DNA recognition by triplex-forming oligonucleotides (TFOs) is usually limited by homopurine-homopyrimidine sequence in duplexes.. Modifications of the third strand may overcome this limitation.. Chimeric alpha-beta TFOs are expected to form triplex DNA upon binding to non-regular sequence duplexes.

Article Title: Non-methylated Genomic Sites Coincidence Cloning (NGSCC): an approach to large scale analysis of hypomethylated CpG patterns at predetermined genomic loci.
Article Snippet: We have developed a new approach to the analysis of hypomethylated CpG patterns within predetermined,megabase long, genome regions.. The approach, which we term Non-methylated Genomic Sites Coincidence Cloning (NGSCC), includes threemain steps.. First, total genomic DNA is digested with a methylation sensitive restriction endonuclease, such as Hpa II or Hha I.

Article Title: Cell line fingerprinting using retroelement insertion polymorphism
Article Snippet: .. Oligonucleotide primers for PCR amplification were synthesized using an ASM-102U DNA synthesizer (Biosset Ltd., Novosibirsk, Russia). .. PCR Amplification PCR amplification of 26 individual L1 and 21 Alu elements was performed in 25-μL reaction volumes that contained 20–50 ng genomic DNA, 10 pmol each of oligonucleotide primers, 125 μM each of dNTPs, 16.6 mM (NH4)2SO4, 67 mM Tris-HCl buffer, pH 8.8, 1.5 mM MgCl2, and 0.5 U Taq DNA polymerase (SibEnzyme, Novosibirsk, Russia).

Article Title: Formation of stable triplexes between purine RNA and pyrimidine oligodeoxyxylonucleotides
Article Snippet: After 1 h incubation at 37°C, the reaction was stopped at –20°C and the cDNA produced was analysed by 8% PAGE, 6.5 M urea in TBE buffer or 1% agarose gel electrophoresis in TAE buffer, followed by autoradiography with a phosphorimager (Storm 840; Molecular Dynamics). .. All oligonucleotides were synthesized on an ASM-102U DNA synthesizer (Biosset, Novosibirsk, Russia). ..

Article Title: Methylation-free site patterns along a 1-Mb locus on Chr19 in cancerous and normal cells are similar. A new fast approach for analyzing unmethylated CCGG sites distribution.
Article Snippet: We describe a newly developed technique for rapid identification of positions of genomic DNA breaks, preexisting or introduced by specific digestion, in particular, by restriction endonucleases (RIDGES).. We applied RIDGES in analyzing unmethylated CCGG sites distribution along a 1-Mb long genome region (D19S208–COX7A1 on chromosome 19) in cancerous and normal lung tissues.. Both tissues were characterized by a profoundly uneven density of unmethylated sites along the fragment.

Article Title: Differences in Brain Transcriptomes of Closely Related Baikal Coregonid Species
Article Snippet: Clone inserts were sequenced with an Amersham Biosciences/Molecular Dynamics MegaBACE 4000 Capillary Sequencer. .. Oligonucleotides were synthesized using an ASM-102U DNA synthesizer (Biosset Ltd., Russia). ..

Oligonucleotide Synthesis:

Article Title: [Genome similarity of Baikal omul and sig].
Article Snippet: Two members of the Baikal sig family, a lake sig ( Coregonus lavaretus baicalensis Dybovsky) and omul ( C. autumnalis migratorius Georgi) are close relatives that diverged from the same ancestor 10–20 thousand years ago.. In this work, we studied genomic polymorphism of these two fish species.. The method of subtractive hybridization (SH) did not reveal the presence of extended sequences in the sig genome and their absence in the omul genome.

Polymerase Chain Reaction:

Article Title: Cell line fingerprinting using retroelement insertion polymorphism
Article Snippet: .. Oligonucleotide primers for PCR amplification were synthesized using an ASM-102U DNA synthesizer (Biosset Ltd., Novosibirsk, Russia). .. PCR Amplification PCR amplification of 26 individual L1 and 21 Alu elements was performed in 25-μL reaction volumes that contained 20–50 ng genomic DNA, 10 pmol each of oligonucleotide primers, 125 μM each of dNTPs, 16.6 mM (NH4)2SO4, 67 mM Tris-HCl buffer, pH 8.8, 1.5 mM MgCl2, and 0.5 U Taq DNA polymerase (SibEnzyme, Novosibirsk, Russia).

Amplification:

Article Title: Cell line fingerprinting using retroelement insertion polymorphism
Article Snippet: .. Oligonucleotide primers for PCR amplification were synthesized using an ASM-102U DNA synthesizer (Biosset Ltd., Novosibirsk, Russia). .. PCR Amplification PCR amplification of 26 individual L1 and 21 Alu elements was performed in 25-μL reaction volumes that contained 20–50 ng genomic DNA, 10 pmol each of oligonucleotide primers, 125 μM each of dNTPs, 16.6 mM (NH4)2SO4, 67 mM Tris-HCl buffer, pH 8.8, 1.5 mM MgCl2, and 0.5 U Taq DNA polymerase (SibEnzyme, Novosibirsk, Russia).



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